@SUMA_Make_Spec_FS¶
++ Running @SUMA_Make_Spec_FS version: 2.2.4
OVERVIEW¶
@SUMA_Make_Spec_FS - prepare for surface viewing in SUMA
This script goes through the following steps:
+ verify existence of necessary programs
(afni, to3d, suma, mris_convert)
+ determine the location of surface and COR files
+ creation of ascii surface files via 'mris_convert'
+ creation of left and right hemisphere SUMA spec files
+ creation of an AFNI dataset from the COR files via 'to3d'
+ creation of AFNI datasets from various .mgz volumes created
by FreeSurfer. The segmentation volumes with aseg in the
name are best viewed in AFNI with the FreeSurfer_Seg_255
colormap. See bottom of @SUMA_FSvolToBRIK -help for more
info.
+ renumbered data sets output, to replace old '*rank*' file data
sets. Also new tissue maps based on FS functions and
labels. Collectively, these are the '*REN*' dsets in the output
directory. (Rank dsets are no longer output by default, from
Nov, 2019; instead, use -make_rank_dsets if really needed.)
+ convenience dsets for afni_proc.py processing with tissue-based
regressors (fs_ap_* files: subset of ventricle and WM maps)
+ all created files are stored in a new SUMA directory
USAGE¶
@SUMA_Make_Spec_FS [options] -sid SUBJECT_ID
examples ('-NIFTI' is really useful-- see below!):
@SUMA_Make_Spec_FS -help
@SUMA_Make_Spec_FS -NIFTI -sid subject1
@SUMA_Make_Spec_FS -NIFTI -fspath subject1/surface_stuff -sid subject1
@SUMA_Make_Spec_FS -NIFTI -sid 3.14159265 -debug 1
OPTIONS¶
-help : show this help information
-debug LEVEL : print debug information along the way
e.g. -debug 1
the default level is 0, max is 2
A debug level of 2 will "set echo", so one can follow the actual
commands executed in the script.
-fs_setup : source $FREESURFER_HOME/SetUpFreeSurfer.csh
This might be useful on OS X, as FreeSurfer uses DYLD_LIBRARY_PATH,
which does not propagate to child shells. Then this program would
require them to source SetUpFreeSurfer.csh from .cshrc, which might
happen too often and could be irrirating.
With -fs_setup, that will happen from within this script, so it is
not necessary to do from the .cshrc file.
-fspath PATH : path to 'surf' and 'orig' directories
e.g. -fspath subject1/surface_info
the default PATH value is './', the current directory
This is generally the location of the 'surf' directory,
though having PATH end in surf is OK. The mri/orig
directory should also be located here.
Note: when this option is provided, all file/path
messages will be with respect to this directory.
-extra_annot_labels L1 L2 ... : convert extra annot files into ROI dsets
e.g. -extra_annot_labels aparc
FS typically outputs annotation files:
lh.aparc.a2005s.annot
rh.aparc.a2005s.annot
lh.aparc.a2009s.annot
rh.aparc.a2009s.annot
from each of which Make_Spec extracts a colormap, ROI and a
displayable surface dataset.
Use this option to specify other labels to extract.
If LABEL is specified, then expected annotation files will be:
lh.LABEL.annot
rh.LABEL.annot
-extra_fs_dsets AA BB CC ...
: FS calculates many types of data on the surface,
listed in their surf/ output directory. By default, this
program brings the following ones into the SUMA surface realm:
thickness curv sulc
which get turned into 'std.141.{l,r}h.curv.niml.dset' and
'{l,r}h.curv.gii.dset' files, for example.
This option allows the user to list *other* dsets to include,
as well. Ones that users have selected are, for example:
volume area area.pial curv.pial
-make_rank_dsets : before Nov 14, 2019, *rank* dsets used to be
created by this command by default; these dsets mapped the
FreeSurfer ROI numbering to a 1..N set of labels, where N
was the number of ROIs. Because this number might *not* be
constant across a group (though, the ROI string labels in
each would be), we don't recommend using these; the *REN*
dsets are renumbered in a consistent, mapped way, and so
those are more useful. The present option is purely for
backward compatability, for Zome Special Scientists out
there who might still use these dsets.
-use_mgz : use MGZ volumes even if COR volumes are there
-neuro : use neurological orientation
e.g. -neuro
the default is radiological orientation
In the default radiological orientation, the subject's
right is on the left side of the image. In the
neurological orientation, left is really left.
* This is not compatible with -NIFTI.
-nocor: This option is no longer supported because it created
GIFTI surfaces with coordinates in RAI, rather than LPI
which is the GIFTI standard. While using RAI surfaces
within AFNI/SUMA is not problematic, the resultant GIFTI
surfaces do not port well to other software.
The replacement option for -nocor is -GNIFTI but the
surfaces will have negated coordinates along the x and y
compared to those with -nocor.
GIFTI surfaces produced with SUMA programs compiled before
August 1st 2013 will have their X and Y coordinates
negated and will no longer line up with the anatomy.
Correcting such surfaces can be done with ConvertSurface
with the following command:
ConvertSurface \
-i lh.smoothwm.gii \
-o_gii lh.smoothwm \
-overwrite \
-xmat_1D NegXY
or for an entire SUMA directory:
cd SUMA
tcsh
foreach ss (*.gii)
ConvertSurface \
-i $ss \
-o_gii $ss \
-overwrite \
-xmat_1D NegXY
end
-GNIFTI/-GIFTI/-IFTI: same as -NIFTI
-NIFTI :Produce files in exchangeable formats. With this option
:COR volumes are no longer used and output volumes
:and surfaces are in alignment with the original
:volume used to create the surface. All volumes are
written out NIFTI format, and all surfaces are
in GIFTI format.
This option is incompatible with -neuro or -use_mgz
** Note: from 22 Feb 2013 through 20 Mar 2017, use of -NIFTI
would distort standard mesh surfaces. To evaluate
effects of this, consider: MapIcosahedron -write_dist.
* If you are seeing this message, that problem was fixed
years ago.
-inflate INF: Create modereately inflated surfaces using
SurfSmooth. INF controls the amount of smoothness
in the final image. It is the number of iterations
in the command such as:
SurfSmooth \
-i lh.white.asc \
-met NN_geom \
-Niter 200 \
-o_gii \
-surf_out lh.inf_200 \
-match_vol 0.01
You can use multiple instances of -inflate to create
inflations of various levels.
-set_space SPACE: Set the space flag of all volumes to
SPACE (orig, MNI, TLRC, MNIa). The default is
orig space.
You should only use this option when the volume you
passed to FreeSurfer was not in 'orig' space.
Use '3dinfo -space YOUR_DATASET' to find the space
of a certain dataset.
-sid SUBJECT_ID : required subject ID for file naming
-ld LD : Create standard mesh surfaces with mesh density
linear depth (see MapIcosahedron -help, option -ld)
set to LD. You can use multiple -ld options.
By default the script will run ld values of 141 and
60.
-ldpref LDpref: Supply what ends up being the -prefix option
for MapIcosahedron. By default it is std.LD.
You need as many -ldpref as you have -ld
-no_ld: Do not run MapIcosahedron.
NOTES¶
Making use of FreeSurfer's -contrasurfreg output with MapIcosahedron:
This script will create SUMA versions of lh.rh.sphere.reg and
rh.lh.sphere.reg but in this current state, MapIcosahedron does
not attempt to use them for backward compatibility.
Should you want to create standard mesh surfaces with node
index correspondence across the hemispheres you will need to run
MapIcosahedron manually in the output SUMA/ directory.
For example:
MapIcosahedron \
-spec SUBJ_rh.spec -ld 60 \
-dset_map rh.thickness.gii.dset \
-dset_map rh.curv.gii.dset \
-dset_map rh.sulc.gii.dset \
-morph rh.lh.sphere.reg.gii \
-prefix std.60.lhreg.
This command is very similar to the one use to create the default
output spec file std.60.SUBJ_rh.spec (look at the top of the spec
file for a record of the command that created it), except for the
last two options -morph and -prefix. By using -morph
rh.lh.sphere.reg.gii the resultant standard-mesh right hemispheres
(std.60.lhreg.rh.*.gii) will have node index correspondence with
std.60.lh.*.gii surfaces. To verify visually the correspondence,
run the following:
count -column 0 36001 > std.60.lh.rh.nodeindex.1D.dset
suma -noniml -spec std.60.SUBJ_lh.spec &
suma -noniml -spec std.60.SUBJ_rh.spec &
suma -noniml -spec std.60.lhreg.SUBJ_rh.spec &
Then load std.60.lh.rh.nodeindex.1D.dset into each of the three SUMA
windows. Note how the color pattern (node indices) matches between
SUBJ_lh and lhreg.SUBJ_rh surfaces, but NOT between SUBJ_lh and
SUBJ_rh surfaces.
COMMENTS¶
0. More help may be found at:
https://afni.nimh.nih.gov/pub/dist/doc/htmldoc/SUMA/main_toc.html
1. Surface file names should look like 'lh.smoothwm'.
2. Patches of surfaces need the word patch in their name, in
order to use the correct option for 'mris_convert'.
3. Flat surfaces must have .flat in their name.
4. You can tailor the script to your needs. Just make sure you
rename it or risk having your modifications overwritten with
the next SUMA version you install.
Authors to pester:
R. Reynolds (reynoldr@mail.nih.gov)
Z. Saad (saadz@mail.nih.gov)
M. Beauchamp (michael.beauchamp@bcm.edu)
