AFNI program: 3dROIMaker
Output of -help
ROIMaker, written by PA Taylor (Nov, 2012), part of FATCAT (Taylor & Saad,
2013) in AFNI.
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THE GENERAL PURPOSE of this code is to create a labelled set of ROIs from
input data. It was predominantly written with a view of aiding the process
of combining functional and tractographic/structural data. Thus, one might
input a brain map (or several, as subbricks) of functional parameters
(e.g., correlation coefficients or ICA maps of Z-scores), set a value
threshold and/or a cluster-volume threshold, and this program will find
distinct ROIs in the data and return a map of them, each labelled with
an integer. One can also provide a reference map so that, for example, in
group studies, each subject would have the same number label for a given
region (i.e., the L motor cortex is always labelled with a `2'). In order
to be prepared for tractographic application, one can also enlarge the
gray matter ROIs so that they intersect with neighboring white matter.
One can either specify a number of voxels with which to pad each ROI,
and/or input a white matter skeleton (such as could be defined from a
segmented T1 image or an FA map) and use this as an additional guide for
inflating the GM ROIs. The output of this program can be used directly
for guiding tractography, such as with 3dTrackID.
If an input dataset ('-inset INSET') already contains integer delineation,
such as using a parcellation method, then you can preserve these integers
*even if the ROIs are contiguous* by using the same set as the reference
set (-> '-refset INSET', as well). Otherwise, contiguous blobs defined
will likely be given a single integer value in the program.
Labeltable functionality is now available. If an input '-refset REFSET'
has a labeltable attached, it will also be attached to the output GM and
inflated GMI datasets by default (if you don't want to do this, you can
use the '-dump_no_labtab' to turn off this functionality). If either no
REFSET is input or it doesn't have a labeltable, one will be made from
zeropadding the GM and GMI map integer values-- this may not add a lot of
information, but it might make for more useful output.
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OUTPUTS:
+ `GM' map of ROIs :based on value- and volume-thresholding, would
correspond most closely to gray matter regions of
activation. The values of each voxel are an integer,
distinct per ROI.
+ `GMI' map of ROIs :map of inflated GM ROIs, based on GM map, with the
ROIs inflated either by a user-designed number of
voxels, or also possibly including information of
the WM skeleton (so that inflation is halted after
encountering WM). The values of each voxel are the
same integers as in the GM map.
+ RUNNING, need to provide:
-inset INSET :3D volume(s) of values, esp. of functionally-derived
quantities like correlation values or ICA Z-scores.
-thresh MINTHR :threshold for values in INSET, used to great ROI
islands from the 3D volume's sea of values.
-prefix PREFIX :prefix of output name, with output files being:
PREFIX_GM* and PREFIX_GMI* (see `Outputs', above).
and can provide:
-refset REFSET :3D (or multi-subbrick) volume containing integer
values with which to label specific GM ROIs after
thresholding. This can be useful to assist in having
similar ROIs across a group labelled with the same
integer in the output GM and GMI maps.
If an INSET ROI has no corresponding REFSET label,
then the former is marked with an integer greater
than the max refset label. If an INSET ROI overlaps
with multiple REFSET ROIs, then the former is split
amongst the latter-- overlap regions get labelled
first, and then REFSET labels grow to cover the INSET
ROI in question. NB: it is possible to utilize
negative-valued ROIs (voxels =-1) to represent NOT-
regions for tracking, for example.
-volthr MINVOL :integer number representing minimum size a cluster of
voxels must have in order to remain a GM ROI after
the values have been thresholded. Number might be
estimated with 3dAlphaSim, or otherwise, to reduce
number of `noisy' clusters.
-only_some_top N :after '-volthr' but before any ref-matching or
inflating, one can restrict each found region
to keep only N voxels with the highest inset values.
(If an ROI has <N voxels, then all would be kept.)
This option can result in unconnected pieces.
-only_conn_top N :similar-ish to preceding option, but instead of just
selecting only N max voxels, do the following
algorithm: start the ROI with the peak voxel; search
the ROI's neighbors for the highest value; add that
voxel to the ROI; continue until either the ROI has
reached N voxels or whole region has been added.
The returned ROI is contiguous and 'locally' maximal
but not necessarily globally so within the original
volume.
-inflate N_INFL :number of voxels which with to pad each found ROI in
order to turn GM ROIs into inflated (GMI) ROIs.
ROIs won't overlap with each other, and a WM skeleton
can also be input to keep ROIs from expanding through
a large amount of WM ~artificially (see below).
-trim_off_wm :switch to trim the INSET to exclude voxels in WM,
by excluding those which overlap an input WM
skeleton, SKEL (see `-wm_skel', below; to trim off
CSF, see separate `-csf_skel'). NB: trimming is done
before volume thresholding the ROIs, so fewer ROIs
might pass, or some input regions might be split
apart creating a greater number of regions.
-wm_skel SKEL :3D volume containing info of WM, as might be defined
from an FA map or anatomical segmentation. Can be
to guide ROI inflation with `-skel_stop'.
-skel_thr THR :if the skeleton is not a mask, one can put in a
threshold value for it, such as having THR=0.2 if
SKEL were a FA map.
-skel_stop :switch to stop inflation at locations which are
already on WM skeleton (default: off; and need
`-wm_skel' to be able to use).
-skel_stop_strict :similar to '-skel_stop', but this also does not
allow any inflation *into* the skel-region. The
'-skel_stop' let's the inflation go one layer
*into* the skel-region, so this is stricter. This
option might be my preference these days.
-csf_skel CSF_SK :similar to SKEL, a 3D volume containing info of CSF.
NB: however, with CSF_SK, info must just be a binary
mask already, and it will only be applied in trimming
procedure (no affect on inflation); if input, INSET
is automatically trimmed of CSF, independent of
using `-trim_off_wm'. Again, trimming done before
volume thresholding, so may decrease/separate regions
(though, that may be useful/more physiological).
-mask MASK :can include a mask within which to apply threshold.
Otherwise, data should be masked already. Guess this
would be useful if the MINTHR were a negative value.
It's also useful to ensure that the output *_GMI*
ROI masks stay within the brain-- this probably won't
often matter too much.
For an N-brick inset, one can input an N- or 1-brick
mask.
-neigh_face_only : **DEPRECATED SWITCH** -> it's now default behavior
to have facewise-only neighbors, in order to be
consistent with the default usage of the clusterize
function in the AFNI window.
-neigh_face_edge :can loosen the definition of neighbors, so that
voxels can share a face or an edge in order to be
grouped into same ROI (AFNI default is that neighbors
share at least one edge).
-neigh_upto_vert :can loosen the definition of neighbors, so that
voxels can be grouped into the same ROI if they share
at least one vertex (see above for default).
-nifti :switch to output *.nii.gz GM and GMI files
(default format is BRIK/HEAD).
-preinfl_inset PSET :as a possible use, one might want to start with a WM
ROI, inflate it to find the nearest GM, then expand
that GM, and subtract away the WM+CSF parts. Requires
use of a '-wm_skel' and '-skel_stop', and replaces
using '-inset'.
The size of initial expansion through WM is entered
using the option below; then WM+CSF is subtracted.
The *_GM+orig* set is returned. In the *_GMI+orig*
set, the number of voxels expanded in GM is set using
the '-inflate' value (WM+CSF is subtracted again
before output).
-preinfl_inflate PN :number of voxels for initial inflation of PSET.
-dump_no_labtab :switch for turning off labeltable attachment to the
output GM and GMI files (from either from a '-refset
REFSET' or from automatic generation from integer
labels.
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+ EXAMPLE:
3dROIMaker \
-inset CORR_VALUES+orig. \
-thresh 0.6 \
-prefix ROI_MAP \
-volthr 100 \
-inflate 2 \
-wm_skel WM_T1+orig. \
-skel_stop_strict
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If you use this program, please reference the introductory/description
paper for the FATCAT toolbox:
Taylor PA, Saad ZS (2013). FATCAT: (An Efficient) Functional
And Tractographic Connectivity Analysis Toolbox. Brain
Connectivity 3(5):523-535.
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