Hi AFNI team (or better: hi Gang),

I would like perform ISC analysis on whole-brain level as well as for a list of a priori defined ROIs. In addition to synchronicity overall, I am also interested in the effects of group and other covariates as well as their interaction. Thus far, I have used 3dISC for whole brain analysis specifying a grey matter mask, thresholded the results using p = 0.001 and cluster-size corrected the resulting map based on the output of 3dClustSim using ACF estimate from the errts.* files as input. Is this an appropriate way of dealing with the multiple testing problem within ISC analysis on whole brain? Likewise, I was wondering whether I can proceed in a similar manner for the ROI analysis specifying the corresponding ROI masks in the 3dISC command?

I also came across your recent publication in NeuroImage on applying a BML framework to the ISC framework. Is this a more appropriate framework for the ROI analysis? Has this been implemented yet as an AFNI program?

I am looking forward to your reply.

Best regards,
Stef