In my experimental design, there are two trial types and we want to time-lock the stimulus to the TR. To create the stimulus schedule, I am running RSFgen in a similar way as described in: [
afni.nimh.nih.gov]. I ran RSFgen specifying 50 'A' trials, 50 'B' trials, and 100 'rest trials'.
When using RSFgen in this way, there are often times when two (or three, or four) trials end up being back-to-back with no rest trials between them; in effect, you occasionally get an ISI=0. Is this not problematic from the perspective of deconvolving the HRF if you end up having trials that are back-to-back like that on occasion? Thank you for your help in clarifying this matter.